Acanthamoeba and Naegleria Culture
Also known as: ACANT
Use
The Acanthamoeba and Naegleria Culture test is used to detect free-living amoeba, specifically Acanthamoeba species and Naegleria fowleri, in various specimen types. This test is clinically significant for diagnosing infections caused by these amoebas, which can lead to serious conditions like primary amoebic meningoencephalitis and Acanthamoeba keratitis, especially when they contaminate water sources and come in contact with susceptible individuals.
Special Instructions
Specimen source is preferred when submitting samples. Additionally, CSF samples should be directed to the Acanthamoeba and Naegleria Culture and Stain, CSF (ARUP test code 3000878). For detection of Entamoeba histolytica, refer to the Entamoeba histolytica Antigen, EIA (ARUP test code 0058001).
Limitations
This culture test will not detect Balamuthia mandrillaris, another free-living amoeba that can cause similar infections. Additionally, specimens in media or preservatives are unacceptable for this test, which may affect the ability to accurately detect the presence of Acanthamoeba spp and Naegleria fowleri. Proper specimen handling and adherence to collection and transport instructions are crucial to ensure test accuracy.
New York Approved
Methodology
Culture-based
Biomarkers
LOINC Codes
- 9781-6
Result Turnaround Time
2-10 days
Related Documents
For more information, please review the documents below
Specimen
Other
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Place corneal scrapings, vitreous fluid, or tissue in 2 mL of Page Amoeba Saline (ARUP supply #31917).
Storage Instructions
Room temperature.
Causes for Rejection
Specimens in media or preservatives.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 72 hours |
| Refrigerated | Unacceptable |
| Frozen | Unacceptable |