Acid-Fast Bacillus (AFB) Identification with Reflex to Susceptibility
Also known as: MC AFBIS
Use
This test is used to identify species within the Mycobacterium tuberculosis complex and determine their susceptibility to various drugs. It assists in diagnosing tuberculosis and differentiating it from related infections by mycobacteria such as M. kansasii, M. avium-intracellulare complex, M. fortuitum complex, M. abscessus complex, M. chelonae, and M. immunogenum. Proper identification and susceptibility testing are crucial for effective treatment and management of these infections.
Special Instructions
If susceptibility testing is desired for pyrazinamide (PZA), consult your local public health laboratory or the CDC for pncA gene analysis. For other first-line drugs like rifampin, isoniazid, and ethambutol, the test is performed by ARUP. Additional charges apply if a significant organism is identified, leading to a susceptibility panel.
Limitations
Mixed cultures or non-viable organisms are unacceptable for this test. It does not cover organisms other than the Mycobacterium species listed. Susceptibility results depend on organism growth, and results from non-standardized methodologies should be interpreted with caution. Certain drug MIC values, like those for ethambutol and rifampin, are not reported as they may not predict clinical response.
New York Approved
Methodology
Mass Spectrometry
Biomarkers
LOINC Codes
- 29579-0
Result Turnaround Time
14 days
Related Documents
For more information, please review the documents below
Specimen
Cultured Cells
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Transport sealed container with pure culture on solid or in liquid media. Place in an individually sealed bag.
Storage Instructions
Room temperature. Follow Infectious Substance Category A shipping guidelines if applicable.
Causes for Rejection
Mixed cultures or non-viable organisms. Organisms submitted on agar plate.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 2 weeks |
| Refrigerated | 2 weeks |
| Frozen | 2 weeks |