Antimicrobial Susceptibility - Surveillance Carbapenemase Gene Detection by PCR
Also known as: CARBA SWAB
Use
This assay detects five carbapenemase gene families (blaKPC, blaNDM, blaOXA-48, blaVIM, blaIMP) encoding enzymes that may confer resistance to carbapenem and other beta-lactam antibiotics. It serves as an aid to infection control in detecting carbapenem-resistant bacteria. It's intended for use in infection control and is not suitable for guiding or monitoring infection treatment.
Special Instructions
Not provided.
Limitations
The assay generates false-negative results for samples containing certain IMP gene sequences. It does not detect resistance mechanisms other than those conferred by the targeted gene families, and negativity can't exclude resistance mechanisms or low-concentration nucleic acids.
Methodology
PCR-based (PCR)
Biomarkers
LOINC Codes
- 49617-4 - blaKPC Islt/Spm Ql
- 73982-1 - blaNDM Islt/Spm Ql
- 85498-4 - blaIMP Islt/Spm Ql
- 85501-5 - blaVIM Islt/Spm Ql
- 85503-1 - blaOXA-48 Islt/Spm Ql
- 48767-8 - Annotation comment Imp
Result Turnaround Time
1-2 days
Related Documents
For more information, please review the documents below
Specimen
Rectal Swab
Volume
Not provided
Minimum Volume
Not provided
Container
eSwab transport media
Collection Instructions
Refer to collection instructions at https://www.aruplab.com/Specimen-Handling/resources/pdf/rectal-eswab.pdf. Transport rectal swab in eSwab transport media (ARUP Supply #45877).
Storage Instructions
Specimen should be kept refrigerated during transport.
Causes for Rejection
Specimen source not provided.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 48 hours |
| Refrigerated | 6 days |
| Frozen | 6 days |
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