Aspergillus Species by PCR
Also known as: ASPERPCR
Use
This test is used for the detection of Aspergillus species in clinical specimens. It specifically detects the most common Aspergillus species and differentiates Aspergillus fumigatus, which is one of the primary causes of invasive aspergillosis. Early detection is crucial as this condition can lead to severe respiratory issues, particularly in immunocompromised individuals.
Special Instructions
Not provided.
Limitations
This test may cross-react with Penicillium species and Paecilomyces species due to the broad range of the pan-Aspergillus primers. A negative result does not rule out the presence of PCR inhibitors in the patient specimen or assay-specific nucleic acid in concentrations below the level of detection by the assay. Therefore, clinical correlation is necessary to interpret the results accurately.
Methodology
PCR-based (PCR)
Biomarkers
Aspergillus fumigatus
MicroorganismAspergillus species
Microorganism
LOINC Codes
- 31208-2 - Specimen source
- 49863-4 - Aspergillus DNA Spec Ql NAA+probe
- 62478-3 - A fumgtus DNA Spec Ql NAA+probe
Result Turnaround Time
2-5 days
Related Documents
For more information, please review the documents below
Specimen
Bronchoalveolar Lavage
Volume
1 mL
Minimum Volume
0.9 mL
Container
Sterile container
Collection Instructions
Transfer 1 mL bronchoalveolar lavage (BAL), bronchial wash, sputum to a sterile container.
Storage Instructions
Keep frozen.
Causes for Rejection
Tissues in optimal cutting temperature compound
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 2 weeks |
| Refrigerated | 2 weeks |
| Frozen | 2 weeks |
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