Bladder Cancer by FISH
Also known as: BC REQUEST
Use
This test aids in the diagnosis of urothelial carcinoma and monitoring for tumor recurrence. It utilizes the UroVysion Bladder Cancer Kit to detect aneuploidy for chromosomes 3, 7, and 17, and/or the loss of the 9p21 locus using fluorescence in situ hybridization (FISH). It is designed to detect genetic abnormalities commonly associated with urothelial cancers, providing critical information for both diagnosis and ongoing management of bladder cancer patients.
Special Instructions
The test requires a second-morning, clean-catch voided urine specimen collected in a PreservCyt collection vial, available via the UroCyte Urine Collection Kit. This kit can be ordered online from eSupply using ARUP Connect or through Client Services. Ensure specimens are transported in PreservCyt fixative. The recommended collection instructions should be followed carefully to ensure accurate results.
Limitations
Negative results indicate a lack of chromosomal abnormalities indicative of urothelial carcinoma. However, negative results do not rule out cancer, particularly in the presence of clinical symptoms suggesting urothelial carcinoma. Further clinical evaluation may be needed in such cases. Positive results suggest the presence of urothelial carcinoma or other urologic malignancies, but confirmatory diagnostics and clinical correlation are necessary to determine the exact source of the cancerous cells. Some urothelial cancers might not be detected if they involve genetic changes outside the scope of this test.
FDA ApprovedNew York Approved
Methodology
Chromosomal / Cytogenetics (FISH)
Biomarkers
LOINC Codes
- 66746-9
- 66746-9
- 8100-0
- 22636-5
- 22634-0
Result Turnaround Time
4-14 days
Related Documents
For more information, please review the documents below
Specimen
Urine
Volume
85 mL
Minimum Volume
35 mL
Container
PreservCyt collection vial
Collection Instructions
Collect a second-morning, clean-catch voided urine specimen using the UroCyte Urine Collection Kit.
Storage Instructions
Store transported specimens at ambient or refrigerated temperatures.
Causes for Rejection
Unfixed specimens not in PreservCyt fixative and frozen specimens are unacceptable. Specimens submitted in expired collection vials are also not accepted.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 1 week from collection |
| Refrigerated | 1 week from collection |
| Frozen | Unacceptable |