Bupropion and Metabolite, Serum or Plasma
Also known as: BUPRO SP
Use
Bupropion is an antidepressant drug indicated for the treatment of major depressive disorder and is also used for smoking cessation. It's predominantly metabolized to hydroxybupropion, which retains about 50% of the parent drug's activity. This test measures the serum levels of bupropion and its metabolite to optimize drug therapy and monitor patient adherence. The therapeutic monitoring is essential due to the influence of drug-drug interactions on CYP2B6 metabolism and the need for dose adjustments in patients with renal or hepatic impairment.
Special Instructions
This test should be ordered to optimize drug therapy and monitor patient adherence with regard to bupropion use. The timing of specimen collection should be a predose (trough) draw at steady state concentration. The specimen should be separated from cells ASAP or within 2 hours of collection and transferred to an ARUP Standard Transport Tube for accurate results.
Limitations
The pharmacokinetics of bupropion and its metabolite can be affected by drug-drug interactions that influence CYP2B6 metabolism, potentially requiring dose adjustments. The assay may not fully account for all interacting factors affecting drug levels and activity. Adverse effects such as seizures, hypertension, and neuropsychiatric issues may occur.
New York Approved
Methodology
Mass Spectrometry
Biomarkers
LOINC Codes
- 6706-6
- 9418-5
Result Turnaround Time
1-8 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
2 mL
Minimum Volume
0.5 mL
Container
Plain red. Also acceptable: Lavender (K2 or K3EDTA), pink (K2EDTA), green (Heparin) or gray (Potassium oxalate or Sodium Fluoride).
Collection Instructions
Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer to an ARUP Standard Transport Tube.
Patient Preparation
Timing of specimen collection: Predose (trough) draw at steady state concentration.
Storage Instructions
CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.
Causes for Rejection
Whole blood. Gel separator tubes, light blue (citrate), or yellow (SPS or ACD solution).
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Unacceptable |
| Refrigerated | Unacceptable |
| Frozen | 14 days |