C4D by Immunohistochemistry
Also known as: C4D IHC
Use
This test is a laboratory-developed immunohistochemistry (IHC) stain service offered by ARUP Laboratories. It detects complement split product C4d deposition in formalin-fixed, paraffin-embedded tissue or cellblock preparations. C4d deposition in capillaries is clinically used as an indicator of antibody-mediated rejection in organ transplant pathology, particularly in kidney and heart allografts.
Special Instructions
Not provided.
Limitations
Frozen, non‑formalin‑fixed, or non‑paraffin‑embedded specimens are unacceptable. Sections must be prepared appropriately; unsuitable specimens include non‑representative tissue type or depleted specimens. Frozen specimens are unacceptable. Stability is indefinite at ambient or refrigerated temperatures; frozen specimens are not acceptable. Interpretation requires a separate consult if pathologist insight is desired.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
LOINC Codes
- 49461-7 - C4d Ag Tiss Ql ImStn
- 94736-6 - Tissue block ID Spec
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Transport tissue block or 5 unstained (3‑ to 5‑micron thick sections), positively charged slides in a tissue transport kit (ARUP supply #47808).
Storage Instructions
Protect from excessive heat; ship in cooled container during summer months.
Causes for Rejection
Non‑representative tissue type; depleted specimens; frozen specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |
Other tests from different labs that may be relevant