CD25 by Immunohistochemistry
Also known as: CD25 IHC
Use
This technical service performs immunohistochemical staining for CD25 (the interleukin‑2 receptor alpha chain) on tissue or cell specimens, facilitating detection of CD25 expression in diagnostic contexts such as hematolymphoid neoplasms. As a stain‑and‑return (technical) assay, it provides the laboratory stain without interpretive diagnostic reporting, enabling downstream pathology evaluation.
Special Instructions
Submit via electronic request or with ARUP Immunohistochemistry Stain Form (#32978) if electronic ordering is unavailable. Specimens must be formalin‑fixed, paraffin‑embedded tissue or cellblock, transported either as a tissue block or at least two unstained positively charged slides (3‑5 µm), avoiding oven‑baking if precut. Use ARUP transport kit #47808, available via ARUP Connect or Client Services. Protect specimens from heat; ship in cooled container during summer. Submission is stain‑only and return technical service.
Limitations
This is a technical (stain and return) only service; it does not include pathologist interpretation or diagnostic reporting. Slides or blocks must contain representative tissue; depleted or non‑representative specimens are unacceptable. Frozen specimens are not acceptable. The service performed Monday through Friday; turnaround may vary based on receipt. No interpretive data or reference interval provided.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
LOINC Codes
- 94736-6
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Formalin fix (10 percent neutral buffered formalin) and paraffin embed specimen (cells must be prepared into a cellblock). Transport tissue block or 5 unstained (3‑ to 5‑micron thick) positively charged slides (minimum 2 slides). Do not oven bake if sending precut slides. Use tissue transport kit (#47808).
Storage Instructions
Protect paraffin block and/or slides from excessive heat; ship in cooled container during summer.
Causes for Rejection
Depleted specimens. Specimens submitted with non‑representative tissue type.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |