CD33 by Immunohistochemistry
Also known as: CD33 IHC
Use
CD33 by Immunohistochemistry (IHC) is utilized to detect the presence and distribution of the CD33 antigen in tissue samples, primarily for diagnostic purposes in hematopathological evaluations. This test is often employed to support the diagnosis of acute myeloid leukemia (AML) where CD33 expression is closely scrutinized. The procedure aids in the differentiation of leukemic blasts and the assessment of potential treatment targets, which can be crucial for guiding therapeutic decisions.
Special Instructions
Not provided.
Limitations
This test is specifically technical and does not include interpretative consultation. Only specimens prepared in formalin fix and paraffin embed are acceptable, and they must not be depleted or contain non-representative tissue. Results from immunohistochemistry are contingent on the preservation quality of specimens and may vary with degradation or improper handling. The test is restricted to providing a stain-only result, which requires subsequent interpretation by qualified pathologists.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
CD33
Protein
LOINC Codes
- 94736-6 - Tissue block ID Spec
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
2 slides
Container
Paraffin block or positively charged slides in a tissue transport kit
Collection Instructions
Formalin fix (10 percent neutral buffered formalin) and paraffin embed specimen. Cells must be prepared into a cellblock.
Storage Instructions
Protect paraffin block and/or slides from excessive heat during transport.
Causes for Rejection
Depleted specimens. Specimens submitted with non-representative tissue type.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |
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