DNA Cell Cycle Analysis - Ploidy and S-Phase
Also known as: DNA MISC
Use
The DNA Cell Cycle Analysis - Ploidy and S-Phase test assists in evaluating the prognosis of malignancy across various tumor specimen types. The analysis provides insights into tumor DNA content which is diagnostically and prognostically significant depending on the tumor type and tissue source. It is particularly valuable in understanding disease-free survival rates and treatment sensitivity in conditions like acute lymphoblastic leukemia (ALL), especially in pediatric patients where the DNA index indicates remission and sensitivity to antimetabolites.
Special Instructions
Not provided.
Limitations
Specimens fixed in certain solutions such as Bouin's solution, mercuric chloride containing fixatives, or ethanol-based fixatives are not acceptable. Also, clotted or hemolyzed blood or bone marrow and decalcified specimens will be rejected. The test does not include specific interpretive information for all tumor types, and the diagnostic and prognostic importance may vary based on the source tissue and type of tumor. The S-Phase information may not be separately available for certain diploid and hypodiploid tumors.
Methodology
Cell-based / Cytometry (Flow Cytometry)
Biomarkers
DNA Content
AnalyteDNA Index
AnalyteS-Phase Percent
Analyte
LOINC Codes
- 11526-1 - Pathology study
- 30911-2 - DNA Ploidy Spec FC
- 30912-0 - DNA Index Spec FC-aCnc
- 31208-2 - Specimen source
- 41294-0 - S Phs Cells Spec Ql
Result Turnaround Time
3-9 days
Related Documents
For more information, please review the documents below
Specimen
Tumor Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Paraffin-embedded tissue block enriched with tumor.
Storage Instructions
Refrigerated or room temperature.
Causes for Rejection
Products of conception, no tumor tissue remaining on block, or specimens fixed in specified unacceptable solutions.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |
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