Donath Landsteiner
Also known as: IRL-DL
Use
The Donath Landsteiner test is used to diagnose paroxysmal cold hemoglobinuria (PCH), the rarest form of autoimmune hemolytic anemia. This condition is most commonly transient in young children secondary to viral infections but can also be associated with syphilis or exist as an idiopathic chronic form, particularly in older adults. This test is crucial in diagnosing PCH, which is characterized by the presence of cold-reacting antibodies that can cause red blood cell destruction upon warming.
Special Instructions
The presence of other red blood cell antibodies may interfere with the test, potentially leading to inconclusive results. It is essential to maintain the specimen at 37 degrees C until the serum is separated from cells. Additionally, the collection must avoid separator or gel tubes as they lead to unacceptable conditions for the test.
Limitations
The test is specifically designed to detect the presence of hemolytic antibodies associated with paroxysmal cold hemoglobinuria. However, the presence of other antibodies may interfere with the results, making them inconclusive. The test requires careful handling of specimens, including maintaining them at 37 degrees C until separation. Gel or separator tubes should not be used, as these are unacceptable for sample integrity.
New York Approved
Methodology
Other
Biomarkers
LOINC Codes
- 18287-3
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
3 mL
Minimum Volume
2 mL
Container
Plain Red
Collection Instructions
Maintain specimen at 37 degrees C until serum is separated from cells. Transport 3 mL serum. (Min: 2 mL)
Storage Instructions
Refrigerated
Causes for Rejection
Separator or gel tubes.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Unacceptable |
| Refrigerated | 1 week |
| Frozen | Unacceptable |