Glomerular Basement Membrane Antibody, IgG by Multiplex Bead Assay and IFA
Also known as: GBM-G PAN
Use
This test is used for detecting glomerular basement membrane (GBM) antibodies in suspected or established anti-GBM disease. The presence of IgG antibodies to GBM antigen, when detected by either indirect fluorescent antibody (IFA) or multiplex bead assay, supports a diagnosis of Goodpasture syndrome. The combined result of both assays during initial evaluation improves the diagnostic sensitivity for disease.
Special Instructions
A positive result in one or both assays should be confirmed by renal biopsy. Collect serum in a serum separator tube. Separate serum from cells as soon as possible or within 2 hours of collection and transfer 1 mL serum to an ARUP Standard Transport Tube.
Limitations
False positive results can occur due to reactivity against other chains of type IV collagen. This test should be used as part of a comprehensive diagnostic evaluation including renal biopsy, which may be essential in suspected Goodpasture disease with renal involvement for diagnostic confirmation and assessment of renal prognosis. The test is developed by ARUP Laboratories and has not been cleared or approved by the US FDA but is performed in a CLIA certified laboratory and intended for clinical purposes.
New York Approved
Methodology
Immunoassay (Multiplex Protein Panel)
Biomarkers
LOINC Codes
- 29994-1
- 44446-3
- 29994-1
- 44446-3
Result Turnaround Time
1-5 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.3 mL
Container
ARUP Standard Transport Tube
Collection Instructions
Separate serum from cells ASAP or within 2 hours of collection.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 48 hours |
| Refrigerated | 2 weeks |
| Frozen | 1 year |