Helicobacter pylori Culture
Also known as: MC HPYL
Use
The Helicobacter pylori Culture test is used for the diagnosis of H. pylori infection, particularly in children or in patients with treatment failure. The culture allows for the growth and identification of the Helicobacter pylori bacteria, providing an opportunity to perform susceptibility testing. This can guide treatment by identifying antibiotics to which the bacteria are sensitive or resistant.
Special Instructions
Specimen source is required, and the specimen must be preserved in Brucella broth with 20% glycerol immediately after collection. The specimen should be refrigerated post-inoculation and stored in accordance with the manufacturer's label until inoculation. Uninoculated transport media should be stored as per manufacturer instructions.
Limitations
Negative culture results do not exclude the possibility of Helicobacter pylori infection. Susceptibility testing and identification of the organism are performed separately and may vary in method. The test has not been cleared or approved by the U.S. Food and Drug Administration but is performed in a CLIA-certified laboratory for clinical purposes.
Methodology
Culture-based
Biomarkers
LOINC Codes
- 587-6
Result Turnaround Time
1-9 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fresh)
Volume
Not provided
Minimum Volume
Not provided
Container
Brucella broth with 20% glycerol
Collection Instructions
Preserve specimen in Brucella broth with 20% glycerol immediately after collection. Also acceptable: Brucella broth, BHI, or equivalent with or without 10-20 percent glycerol.
Storage Instructions
Store uninoculated transport media per manufacturer label. After inoculation, hold refrigerated.
Causes for Rejection
Fecal specimens or swabs.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Unacceptable |
| Refrigerated | 48 hours |
| Frozen | Unacceptable |
