Herpes Simplex Virus Culture
Also known as: V HSVC
Use
The Herpes Simplex Virus (HSV) Culture is a traditional gold-standard test for identifying acute HSV infection in active lesions such as vesicles, ulcers, and inflamed mucous membranes. It is primarily used for diagnostic purposes in cases where HSV infection is suspected, providing critical information for the management and treatment of the condition. While molecular testing is generally preferred due to its sensitivity, the culture method is still a valuable tool for detecting the presence of HSV in clinical specimens.
Special Instructions
This test is approved for use in New York state. The specimen source is preferred for accurate test results. For adequate specimen processing, the collected specimen should be placed in 3 mL of viral transport media and stored refrigerated. This test does not identify orthopoxviruses, including monkeypox virus.
Limitations
The HSV Culture test does not identify orthopoxviruses such as monkeypox virus. It is not applicable for blood, CSF, plasma, or serum specimens, nor for bacterial or molecular transport systems. Calcium alginate, dry, or wood swabs are also unsuitable for this test. The stability of the specimen is limited to 2 hours at ambient temperature and 72 hours when refrigerated; frozen specimens are unacceptable.
New York Approved
Methodology
Cell-based / Cytometry
Biomarkers
LOINC Codes
- 16291-7
Result Turnaround Time
1-5 days
Related Documents
For more information, please review the documents below
Specimen
Other
Volume
3 mL
Minimum Volume
0.5 mL
Container
sterile container
Collection Instructions
For fluid specimens, transfer the specimen to a sterile container or 3 mL viral transport media (ARUP Supply #12884). For swab or tissue specimens, place in 3 mL viral transport media.
Storage Instructions
Store refrigerated. Specimen stability is 2 hours ambient, 72 hours refrigerated.
Causes for Rejection
Blood, CSF, plasma, or serum. Bacterial transport systems; molecular transport systems; calcium alginate, dry, or wood swabs.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 2 hours |
| Refrigerated | 72 hours |
| Frozen | Unacceptable |