HLA-B27
Also known as: HLAB27
Use
HLA-B27 is a serologically-defined allele of the human HLA-B locus. The presence of the HLA-B27 antigen is strongly associated with ankylosing spondylitis and related disorders. It is not a diagnostic test for ankylosing spondylitis, juvenile rheumatoid arthritis, or Reiter syndrome but may assist in diagnosing these conditions if other clinical signs and symptoms are present.
Special Instructions
Specimens must be analyzed within 72 hours of collection. Indeterminate results may require genotype testing to confirm positive HLA-B27 expression. Order Ankylosing Spondylitis (HLA-B27) Genotyping if necessary.
Limitations
The Anti-HLA-B27 antibody (clone GS145.2) used in the assay can cross-react with some other HLA-B locus antigens, potentially creating false-positive results for HLA-B27 expression. Therefore, results within the indeterminate zone will include a recommendation for genotype testing.
New York Approved
Methodology
Cell-based / Cytometry (Flow Cytometry)
Biomarkers
LOINC Codes
- 26028-1
Result Turnaround Time
1-2 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
4 mL
Minimum Volume
0.5 mL
Container
Lavender Hemogard (EDTA), pink Hemogard (K2EDTA), or green Hemogard (sodium or lithium heparin)
Storage Instructions
CRITICAL ROOM TEMPERATURE.
Causes for Rejection
Frozen or refrigerated specimens, specimens older than 72 hours, clotted or hemolyzed specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 72 hours |
| Refrigerated | Unacceptable |
| Frozen | Unacceptable |