HLA Class I Panel (ABC) by Next Generation Sequencing
Use
The HLA Class I Panel (ABC) by Next Generation Sequencing is intended for pretransplant allele matching for transplant candidates and their donors. It is designed to identify HLA-A, HLA-B, and HLA-C allelic polymorphisms, providing critical information for matching donors and recipients in transplant medicine. This test is critical for ensuring compatibility and improving graft survival rates in transplant recipients.
Special Instructions
Not provided.
Limitations
The test has a high analytical sensitivity and specificity of over 99 percent, but rare diagnostic errors can occur due to primer site mutations. There may also be unknown DNA polymorphisms outside of the regions analyzed, whose significance is not currently known. Furthermore, the test does not have New York state approval, and specimens from New York clients need to be sent to a New York state-approved laboratory.
Methodology
NGS (Targeted)
Biomarkers
No genes
Gene
Result Turnaround Time
8-15 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
4 mL
Minimum Volume
1 mL
Container
ARUP Standard Transport Tube
Collection Instructions
Collect specimen using Lavender (K2 EDTA) or Yellow (ACD Solution A) tubes.
Storage Instructions
Refrigerated transport.
Causes for Rejection
Specimens collected in Yellow (ACD Solution B). Clotted, grossly hemolyzed, or heparinized specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 72 hours |
| Refrigerated | 1 week |
| Frozen | Unacceptable |
Other tests from different labs that may be relevant