HLA Class II Panel (DRB1, DQA1 and DQB1) by Next Generation Sequencing
Also known as: HLACLASSII
Use
This test is utilized to identify allelic polymorphisms of HLA-DRB1, DQA1, and DQB1 loci in specimens from transplant candidates and their donors. It is not intended for diagnosing or screening for HLA-related diseases such as celiac disease, narcolepsy, or rheumatologic diseases. The test provides results with HLA locus denotation followed by a two-field four-digit assigned allele, aiding in the compatibility assessment in transplant scenarios.
Special Instructions
The test should not be regarded as investigational or for research purposes and is certified under the Clinical Laboratory Improvement Amendments of 1988 (CLIA-88) for high complexity clinical laboratory testing. Specimens from New York clients will be processed at a New York state-approved laboratory.
Limitations
The assay has a sensitivity and specificity of over 99% for detecting allelic polymorphisms. However, diagnostic errors may occur due to primer site mutations or unknown DNA polymorphisms outside of analyzed regions. The NMDP code indicates possible rare alleles that cannot be excluded, and additional uncharacterized DNA polymorphisms outside analyzed regions could exist.
Methodology
NGS (Targeted)
Biomarkers
Result Turnaround Time
8-15 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
4 mL
Minimum Volume
1 mL
Container
ARUP Standard Transport Tube
Collection Instructions
Transfer blood collected in Lavender (K2 EDTA) or Yellow (ACD Solution A) tubes.
Storage Instructions
Refrigerated.
Causes for Rejection
Specimens collected in Yellow (ACD Solution B). Clotted, grossly hemolyzed, or heparinized specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 72 hours |
| Refrigerated | 1 week |
| Frozen | Unacceptable |