Insulin, 180 Minutes
Also known as: INSULIN180
Use
The Insulin, 180 Minutes test measures the level of insulin in the blood, which is essential in diagnosing and managing conditions like diabetes and insulin resistance. It helps assess the body's insulin response after an oral glucose challenge, typically a 75 g glucose dose. This test is valuable for understanding insulin secretion and action in the setting of glucose metabolism disorders.
Special Instructions
This test reacts on a nearly equimolar basis with the insulin analogs insulin aspart, insulin glargine, and insulin lispro. Insulin detemir exhibits approximately 50% cross-reactivity, whereas insulin glulisine shows negligible reactivity (<3%). The reference interval is established based on a 75 g glucose challenge test. To convert insulin levels from µIU/mL to pmol/L, multiply by 6.0.
Limitations
This test may not accurately reflect insulin levels in patients who have been administered non-standard insulin analogs or formulations. Cross-reactivity with certain insulin analogs, particularly insulin detemir and insulin glulisine, could affect results, reducing test accuracy. It is not suitable for individuals who have not undergone the standardized glucose challenge protocol as expected.
FDA ApprovedNew York Approved
Methodology
Immunoassay (CLIA)
Biomarkers
LOINC Codes
- 27828-3
Result Turnaround Time
1 day
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.4 mL
Container
ARUP Standard Transport Tube
Collection Instructions
Allow specimen to clot completely at room temperature. Separate from cells ASAP or within 2 hours of collection.
Causes for Rejection
Heparinized plasma, I.V. fluid, or Vitreous fluid. Gray (sodium fluoride/potassium oxalate). Hemolyzed specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 8 hours |
| Refrigerated | 1 week |
| Frozen | 1 month |