Kappa/Lambda Light Chain Panel by in situ Hybridization on Paraffin
Also known as: K/L P ISH
Use
The Kappa/Lambda Light Chain Panel by in situ Hybridization is used to quantify kappa and lambda light chains in paraffin-embedded tissues. It aids in the diagnosis and classification of B-cell lymphoproliferative disorders and plasma cell neoplasms by assessing the clonality of light chain expression. The presence of a monoclonal population may indicate a neoplastic process, whereas a polyclonal population is typically associated with reactive conditions.
Special Instructions
Include surgical pathology report when submitting specimens. In cases where multiple specimens are sent, specify if the pathologist should select the most appropriate block for testing, or provide individual orders for each sample. A Pathologist Block Selection Fee may apply if selection guidance is not provided.
Limitations
This test was developed and its performance characteristics were determined by ARUP Laboratories and has not been cleared or approved by the FDA. It should be performed in a CLIA certified laboratory. The test is not intended to detect all possible genetic mutations in the immunoglobulin molecules and may not detect low levels of kappa or lambda mRNA. Proper specimen handling is critical to ensure accuracy of test results.
New York Approved
Methodology
Chromosomal / Cytogenetics (ISH)
Biomarkers
Result Turnaround Time
2-5 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
5 slides
Container
tissue transport kit (ARUP supply #47808)
Collection Instructions
Collect tissue and fix in 10 percent neutral buffered formalin. Paraffin-embed tissue block or prepare 10 unstained 5 micron slides. Transport in a tissue transport kit, obtainable through eSupply using ARUP Connect or by contacting ARUP Client Services.
Storage Instructions
Protect paraffin block and/or slides from excessive heat. Ship in cooled container during summer months.
Causes for Rejection
Paraffin block with no tumor tissue remaining. Specimens fixed in any fixative other than 10 percent neutral buffered formalin. Decalcified specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |