Kappa/Lambda Light Chain Panel by In Situ Hybridization Stain Only
Also known as: SO KLP ISH
Use
The test is used to quantify kappa and lambda light chains in paraffin tissue. These light chains are part of the immunoglobulin molecules, and their measurement can help in the assessment of monoclonal gammopathies and other related disorders. By evaluating the kappa/lambda ratios, clinicians can distinguish between polyclonal and monoclonal proliferation, which is useful in diagnosing and monitoring conditions such as multiple myeloma and other lymphoproliferative disorders.
Special Instructions
IMMUNOHISTOCHEMISTRY ORDERING AND SUBMISSION DETAILS: Submit electronic request. If electronic ordering is unavailable, use an ARUP Immunohistochemistry Stain Form (#32978) with an ARUP client number. The specimen must be formalin-fixed and paraffin-embedded. Avoid oven baking of precut slides. Transport in a tissue transport kit. For additional technical details, contact ARUP Client Services.
Limitations
The test does not include interpretation of the kappa/lambda light chains quantification results. It's vital to pair results with clinical findings and other diagnostic tests for comprehensive evaluation. The test is not FDA approved but is performed in a CLIA-certified laboratory. Specimens that are non-representative or depleted are unacceptable for analysis. Frozen specimens are also unacceptable, as stability is only guaranteed under ambient or refrigerated conditions.
New York Approved
Methodology
Chromosomal / Cytogenetics (ISH)
Biomarkers
LOINC Codes
- 94736-6
Result Turnaround Time
2-5 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
4 slides
Container
Tissue transport kit (ARUP supply #47808)
Collection Instructions
Formalin fix (10 percent neutral buffered formalin) and paraffin-embed specimen. Protect paraffin block and/or slides from excessive heat. Transport tissue block or 8 unstained (3- to 5-micron thick sections), positively-charged slides.
Storage Instructions
Room temperature. Also acceptable: Refrigerated. Ship in cooled container during summer months.
Causes for Rejection
Specimens submitted with non-representative tissue type. Depleted specimens. Frozen specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |