Lymphocyte Subset Panel 2 - CD4 Percent and Absolute
Also known as: CD4
Use
The measurement of CD4 T-cell levels has been used to establish decision points for initiating P. jirovecii prophylaxis, antiviral therapy, and to monitor the efficacy of treatment. CD4 T-cell levels are a criterion for categorizing HIV-related clinical conditions by the CDC's classification system for HIV infection. The Public Health Service has recommended that CD4 T-cell levels be monitored every 3 to 6 months in all HIV-infected persons.
Special Instructions
This test is designed for enumerating the percent and absolute cell count of CD4 Helper T-cells in lysed whole blood. Specimens must be analyzed within stability times provided. Some medications may affect immunophenotyping results and should be listed on the patient test request form. Whole blood is added to fluorochrome-labeled antibodies that bind specifically to cell surface antigens on lymphocytes.
Limitations
The test has not been cleared or approved by the US Food and Drug Administration but was developed and its performance characteristics determined by ARUP Laboratories. It is performed in a CLIA certified laboratory and is intended for clinical purposes. Additional CBC data is not required for the test.
New York Approved
Methodology
Cell-based / Cytometry (Flow Cytometry)
Biomarkers
LOINC Codes
- 45265-6
- 24467-3
- 8123-2
Result Turnaround Time
1-2 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
4 mL
Minimum Volume
0.5 mL
Container
Lavender (EDTA), pink (K2EDTA), or green (sodium or lithium heparin) Hemogard tubes are preferred for laboratory automation and safety.
Storage Instructions
CRITICAL ROOM TEMPERATURE.
Causes for Rejection
Clotted or hemolyzed
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 72 hours (EDTA), 48 hours (Heparin) |