Lymphocyte Subset Panel 4 - T-Cell Subsets Percent and Absolute, Whole Blood
Also known as: TSHORT
Use
The Lymphocyte Subset Panel 4 test is used for monitoring the immune status of HIV-infected patients and is particularly useful for decision-making regarding the initiation of prophylaxis and antiviral therapy. CD4 T-cell levels, which are measured in this test, serve as important markers for evaluating the progression of HIV and determining treatment efficacy. The test is performed to establish decision points for initiating interventions such as P. jirovecii prophylaxis, antiviral therapy, and monitoring the efficacy of treatments. Regular monitoring, as recommended by the Public Health Service, aids in the longitudinal assessment of HIV-infected patients, helping to inform treatment decisions and improve patient management.
Special Instructions
The test is designed to enumerate the percentages and absolute counts of lymphocyte subsets in lysed whole blood. Sample collections for longitudinal studies should be conducted at the same time of day to reduce variability. Specimens must be analyzed within stability times provided, and information about medications that could affect immunophenotyping results should be included on the patient test request form.
Limitations
This test was developed and its performance characteristics were determined by ARUP Laboratories. It has not been FDA cleared or approved, but is performed in a CLIA certified laboratory for clinical purposes. Results could be affected by specimen conditions like clotted or hemolyzed samples, as well as medications not disclosed prior to testing. The test requires critical handling at room temperature and stability varies depending on the anticoagulant used. Additionally, the test is not suitable for BAL specimens and some other condition exclusions may apply.
New York Approved
Methodology
Cell-based / Cytometry (Flow Cytometry)
Biomarkers
LOINC Codes
- 48767-8
- 8122-4
- 20599-7
- 24467-3
- 14135-8
- 8123-2
- 32518-3
- 54218-3
Result Turnaround Time
1-2 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
4 mL
Minimum Volume
0.5 mL
Container
Lavender (EDTA), pink (K2EDTA), or green (sodium or lithium heparin) Hemogard tubes
Storage Instructions
CRITICAL ROOM TEMPERATURE.
Causes for Rejection
BAL specimens, clotted or hemolyzed specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | EDTA: Ambient: 72 hours; Heparin: Ambient: 48 hours |
| Refrigerated | Unacceptable |
| Frozen | Unacceptable |