Lymphoid Enhancing Factor 1 by Immunohistochemistry

Casandra

Casandra Test Code AR73396Version 1 (DRAFT)

Performing Lab

Lab ARUP LaboratoriesLab Test ID 3002539CPT Code 88342

Lymphoid Enhancing Factor 1 by Immunohistochemistry

Also known as: LEF1 IHC

Clinical Use

Order Test

Use

Lymphoid Enhancing Factor 1 (LEF1) immunohistochemistry is used as an immunohistochemical stain (technical component only) to detect LEF1 protein expression in formalin‑fixed, paraffin‑embedded tissue specimens. This stain aids in the evaluation of lymphoid neoplasms by identifying LEF1 expression. Interpretation should be done by a qualified pathologist in conjunction with clinical history and other diagnostic tests. This is a technical‑only service (“stain and return”) and does not include pathologist interpretation.

Special Instructions

Not provided.

Limitations

Specimens must be representative tissue; depleted specimens are unacceptable. Frozen specimens are unacceptable. If sending precut slides, do not oven bake. Interpretation is not included; pathologist consultation must be ordered separately. Slides should be used within recommended stability period. Technical staining only – no diagnostic interpretation is provided.

Test Details

New York Approved

Methodology

Immunoassay (IHC)

Biomarkers

LEF1

Protein

IHC

Expression • Ordinal Score (e.g., TPS, CPS, H-score)

Result Turnaround Time

1-3 days

Specimen

Tissue (FFPE)

Volume

Not provided

Minimum Volume

Not provided

Container

tissue block or unstained slides (min 2, 3–5 μm thick, positively charged)

Collection Instructions

Formalin fix (10 percent neutral buffered formalin) and paraffin embed specimen (cells must be prepared into a cellblock). Protect paraffin block and/or slides from excessive heat. Transport tissue block or 5 unstained (3‑ to 5‑micron thick sections), positively charged slides in a tissue transport kit (ARUP supply #47808); if sending precut slides, do not oven bake.