Microbial Cell-Free DNA by Sequencing (Karius Test)
Also known as: KARIUS
Use
The Microbial Cell-Free DNA by Sequencing (Karius Test) is used for the detection and identification of microbial cell-free DNA from plasma. It is particularly useful in diagnosing infections by identifying the presence of microbial DNA sequences in patient plasma, providing a non-invasive method to detect bloodstream infections, and facilitating pathogen detection via next-generation sequencing (NGS) approaches. The test aids in identifying multiple pathogens, including those responsible for bloodstream infections, and optimizes patient treatment by determining antimicrobial resistance markers.
Special Instructions
Not provided.
Limitations
The test utilizes quantitative massively parallel sequencing which may not detect very low abundance of microbial DNA beyond the analytical sensitivity of the method. The accuracy of pathogen detection is limited to those organisms represented in the database and may not detect rare or novel pathogens. Results might be affected by contamination or improper sample handling.
New York Approved
Methodology
NGS (Targeted)
Biomarkers
LOINC Codes
- 11502-2 - Laboratory report
Result Turnaround Time
3-6 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
2 mL
Minimum Volume
0.7 mL
Container
Plasma preparation tube (PPT) or Lavender (K2EDTA) tube
Collection Instructions
For Plasma preparation tube (PPT): Separate from cells within 6 hours of collection and freeze immediately. Transport in the original tube. For Lavender (K2EDTA): Separate from cells within 24 hours of collection and transfer plasma to a sterile transport tube.
Storage Instructions
CRITICAL FROZEN
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 96 hours |
| Refrigerated | Unacceptable |
| Frozen | 6 months |