NR4A3 Rearrangement by FISH
Also known as: NR4A3 FISH
Use
The NR4A3 Rearrangement by FISH test is designed to detect rearrangements involving the NR4A3 gene. This testing is useful for diagnosing extraskeletal myxoid chondrosarcoma and salivary acinic cell carcinoma. The identification of a rearrangement of the NR4A3 gene locus can assist in the confirmation of these diagnoses. Furthermore, NR4A3 rearrangements may be detected rarely in other tumors, making this test a valuable diagnostic tool for identifying NR4A3 rearranged tumors at an early stage.
Special Instructions
Not provided.
Limitations
This test detects rearrangements involving the NR4A3 gene but does not identify any specific partner genes involved in the rearrangement. Only abnormal signal patterns presented in 25 percent or more of the tumor cells evaluated are considered a positive result. The test does not detect cryptic rearrangements and has not been cleared or approved by the FDA. Correlation with histopathological and clinical findings is essential for a comprehensive interpretation.
New York Approved
Methodology
Chromosomal / Cytogenetics (FISH)
Biomarkers
No genes
Gene
LOINC Codes
- 103558-3 - NR4A3 gene Rear Bld/T FISH
- 81304-8 - Struct var analysis method
- 78236-7 - Cells.IGH rear/Cells cnt NFr Bld/T FISH
- 94736-6 - Tissue block ID Spec
- 31208-2 - Specimen source
Result Turnaround Time
3-7 days
Related Documents
For more information, please review the documents below
Specimen
Tumor Tissue (FFPE)
Volume
Not provided
Minimum Volume
5 unstained (4-micron thick sections) or 1 tissue block
Container
Tissue transport kit
Collection Instructions
Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Protect paraffin block from excessive heat.
Storage Instructions
Room temperature. Refrigerated shipping is acceptable during summer.
Causes for Rejection
Specimens fixed or processed in alternative fixatives or heavy metal fixatives. No tumor in tissue or decalcified specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |