Orthopoxvirus (includes monkeypox virus) by PCR
Also known as: OPOXPCR
Use
This test is used as a sensitive method to detect members of the orthopoxviruses, including monkeypox virus and vaccinia virus. In the United States, a detected result is most likely due to monkeypox virus or vaccinia virus. Other orthopoxviruses may be considered if appropriate. For additional confirmatory testing, the US Centers for Disease Control and Prevention should be referred.
Special Instructions
Not provided.
Limitations
This assay does not differentiate between members of the orthopoxviruses. High viral titer variola virus (smallpox) infections could be detected by this assay, though smallpox was declared eradicated in 1980 by the WHO. A negative result does not rule out the presence of PCR inhibitors in the patient specimen or nucleic acid in concentrations below the level of detection by the assay. It has not been cleared or approved by the US FDA. It's performed in a CLIA certified laboratory and is intended for clinical purposes.
New York Approved
Methodology
PCR-based (PCR)
Biomarkers
Orthopoxvirus
Microorganism
LOINC Codes
- 31208-2 - Specimen source
- 41853-3 - Orthopoxvirus DNA Spec Ql NAA+probe
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Wound Swab
Volume
Not provided
Minimum Volume
Not provided
Container
Viral Transport Media
Collection Instructions
Swab the lesion vigorously to collect adequate DNA. It is not necessary to deroof the lesion before swabbing.
Storage Instructions
Frozen
Causes for Rejection
Calcium alginate swab, wooden swab. Specimens without swabs.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 24 hours |
| Refrigerated | 7 days |
| Frozen | 1 month |