p16 by Immunohistochemistry

Casandra

Casandra Test Code AR22512Version 1 (DRAFT)

Performing Lab

Lab ARUP LaboratoriesLab Test ID 2004064CPT Code 88342

p16 by Immunohistochemistry

Also known as: P16 IHC

Clinical Use

Order Test

Use

p16 (also known as p16 IHC or P16 immunostaining) is used as an immunohistochemical stain (technical service only) to detect expression of the p16 protein, typically in the evaluation of lesions associated with human papillomavirus (HPV)–related neoplasia. It serves as a marker of cell cycle dysregulation, aiding in diagnostic contexts of dysplasia or carcinoma in which p16 overexpression is clinically significant.

Special Instructions

Specimens must be formalin‑fixed (10% neutral buffered formalin) and paraffin‑embedded; cells must be prepared into a cellblock. Transport either the tissue block or at least 5 unstained, positively charged slides (3‑ to 5‑micron thick), minimum of 2 slides. Do not oven bake precut slides. Use ARUP tissue transport kit (#47808). Specimens stored ambient or refrigerated; ship in cooled containers during summer. Submit via electronic request or use ARUP Immunohistochemistry Stain Form (#32978) if needed. Block and slides should be protected from excessive heat. This test is performed as a stain and return (technical only) service. For interface builds, see Interface Map. Alias names include "p16 IHC" and "P16 immunostaining."

Limitations

Unacceptable specimens include non‑representative tissue types and depleted specimens. Frozen specimens are unacceptable. The stain is a technical service only and does not include interpretation unless separately ordered. No interpretive report is provided as part of this technical stain service.

Test Details

New York Approved

Methodology

Immunoassay (IHC)

Biomarkers

2 components

p16 by IHC • IHCp16 Reference Number • IHC

Result Turnaround Time

1-3 days

Specimen

Tissue (FFPE)

Volume

Not provided

Minimum Volume

Not provided

Collection Instructions

Tissue or cells: formalin‑fixed (10 percent neutral buffered formalin) and paraffin embedded; cells prepared into a cellblock. Transport tissue block or 5 unstained (3‑ to 5‑micron thick) positively charged slides, minimum 2 slides. If sending precut slides, do not oven bake.