p16 by Immunohistochemistry
Also known as: P16 IHC
Use
p16 (also known as p16 IHC or P16 immunostaining) is used as an immunohistochemical stain (technical service only) to detect expression of the p16 protein, typically in the evaluation of lesions associated with human papillomavirus (HPV)–related neoplasia. It serves as a marker of cell cycle dysregulation, aiding in diagnostic contexts of dysplasia or carcinoma in which p16 overexpression is clinically significant.
Special Instructions
Not provided.
Limitations
Unacceptable specimens include non‑representative tissue types and depleted specimens. Frozen specimens are unacceptable. The stain is a technical service only and does not include interpretation unless separately ordered. No interpretive report is provided as part of this technical stain service.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Tissue or cells: formalin‑fixed (10 percent neutral buffered formalin) and paraffin embedded; cells prepared into a cellblock. Transport tissue block or 5 unstained (3‑ to 5‑micron thick) positively charged slides, minimum 2 slides. If sending precut slides, do not oven bake.
Storage Instructions
Protect block and/or slides from excessive heat; ambient or refrigerated storage; ship in cooled container during summer months.
Causes for Rejection
Non‑representative tissue type; depleted specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |
Other tests from different labs that may be relevant