p53 by Immunohistochemistry
Also known as: P53 IHC
Use
This IHC stain is used to evaluate p53 protein expression in tissue or cellblock specimens, serving as a marker for aberrant p53 (tumor protein 53) expression. It is performed as a technical stain with pathologist interpretation to assess tumor cells.
Special Instructions
Submit electronic request or use ARUP Immunohistochemistry Stain Form (#32978) if electronic ordering is unavailable. Submit surgical pathology report with specimen. Tissue transport kit (ARUP supply #47808) available via ARUP Connect. Protect paraffin blocks/slides from heat; if sending precut slides, do not oven bake.
Limitations
Unacceptable specimens include non-representative tissue and depleted specimens. Frozen specimens are unacceptable. Protect specimens from excessive heat. Submission without interpretive context or pathology report may limit interpretation.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
5 slides (min: 2 slides noted, but standard offering 5 unstained sections)
Container
tissue block or positively charged slides in tissue transport kit (#47808)
Collection Instructions
Formalin fix (10 percent neutral buffered formalin) and paraffin embed; prepare cellblock if cells. Transport block or 5 unstained 3–5 µm thick positively charged slides. Do not oven bake if precut.
Storage Instructions
Room temperature or refrigerated; ship in cooled container during summer months.
Causes for Rejection
Non-representative tissue; depleted specimens; specimens fixed in other than 10% neutral buffered formalin (as noted in related tests) or frozen
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |