PanFungal Identification by Sequencing
Also known as: PANFUNGSEQ
Use
The PanFungal Identification by Sequencing test is used for the direct detection and identification of fungi in tissue, cerebrospinal fluid, or other body fluids. This assay detects and identifies human fungal pathogens by Sanger sequencing, providing information that assists in clinical diagnosis and management. It is crucial for differentiating between environmental fungal DNA and invasive fungal infections.
Special Instructions
This test is not approved by New York state. Submission of a Non-Permitted Laboratory Request Form to the NYDOH is required prior to specimen collection. Upon NYDOH approval, and if the sample is received at ARUP, testing will be performed.
Limitations
This assay cannot differentiate invasive fungal infection from environmental fungal DNA, hence clinical correlation of sequencing results is recommended. It is important to note that false negatives may occur due to PCR inhibitors within the specimen, low levels of fungal DNA, or mixtures of different fungal sequences.
Methodology
Sanger
Biomarkers
LOINC Codes
- 48767-8
Result Turnaround Time
3-5 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fresh)
Volume
Not provided
Minimum Volume
25 mg
Container
Sterile container
Collection Instructions
Collect fresh tissue; formalin-fixed paraffin-embedded tissue (FFPE); CSF; pleural, abdominal, synovial, peritoneal, and vitreous fluid. Transfer CSF, tissue, and body fluid specimen into a sterile container and freeze immediately.
Storage Instructions
Fresh Tissue: Frozen; FFPE: Room temperature; CSF: Frozen; Body Fluid: Frozen.
Causes for Rejection
Fingernails, toenails, or bone. Formalin-fixed paraffin-embedded tissue on slides. All respiratory specimens such as bronchoalveolar lavage (BAL), sputum, etc.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 5 days |
| Refrigerated | 5 days |
| Frozen | 5 days |