Paroxysmal Nocturnal Hemoglobinuria (PNH), High Sensitivity, RBC and WBC
Also known as: PNH PAN
Use
This test is preferred for the initial diagnosis of paroxysmal nocturnal hemoglobinuria (PNH) and for quantifying PNH clones. It is developed according to published guidelines and includes high-sensitivity testing for WBC and RBC with specific markers like FLAER and CD157. The test measures the most accurate size of the PNH clone using WBC analysis and provides quantification of Type II and Type III RBC clones. It helps in the management of PNH by identifying subclinical PNH populations in myelodysplastic bone marrow disorders, which may correlate with immunotherapeutic response.
Special Instructions
If more than 1% PNH RBCs are detected, a PNH RBC TYPE reflex test will be added at no additional charge. Specimens must be analyzed within the stability times provided.
Limitations
The test's performance may be affected by recent RBC transfusions, which can decrease the percentage of PNH cells measured in RBCs. The WBC assay sensitivity will be much lower for severely pancytopenic patients. The testing frequency may vary depending on the clinical and hematologic parameters of the patient.
New York Approved
Methodology
Cell-based / Cytometry (Flow Cytometry)
Biomarkers
LOINC Codes
- 33662-8
- 77948-8
- 93479-4
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
4 mL
Minimum Volume
4 mL
Container
Lavender (EDTA), pink (K2EDTA), or green (sodium or lithium heparin)
Storage Instructions
Refrigerated.
Causes for Rejection
Clotted or hemolyzed specimens
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 24 hours |
| Refrigerated | 72 hours |
| Frozen | Unacceptable |