Platelet Surface Glycoprotein Expression (PGE) by Flow Cytometry, Whole Blood
Also known as: PGE
Use
This test is used to diagnose Bernard-Soulier syndrome or Glanzmann thrombasthenia in patients with a lifelong history of platelet-type bleeding. It evaluates the expression of platelet surface glycoproteins, including GPIb (CD42b) and GPIIb/IIIa (CD41/CD61), to assess possible deficiencies or abnormalities associated with these conditions.
Special Instructions
Not provided.
Limitations
The test alone cannot confirm asymptomatic carriers of Bernard-Soulier syndrome or Glanzmann thrombasthenia. Further sequence analysis is recommended if a carrier state is suspected. Platelet transfusions prior to testing can artificially increase normal platelet levels, affecting results. Hence, results should be interpreted with caution if the patient has recently received a platelet transfusion.
New York Approved
Methodology
Cell-based / Cytometry (Flow Cytometry)
Biomarkers
GPIb (CD42b)
ProteinGPIIb/IIIa (CD41/CD61)
Protein
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
4 mL
Minimum Volume
0.1 mL
Container
Lavender (EDTA), pink (K2EDTA), or yellow (ACD solution B)
Storage Instructions
Transport at room temperature or refrigerated.
Causes for Rejection
Clotted, hemolyzed, or frozen specimens; specimens older than 72 hours.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 72 hours |
| Refrigerated | 72 hours |
| Frozen | Unacceptable |
Other tests from different labs that may be relevant