Pneumocystis jirovecii by PCR
Also known as: PCP PCR
Use
This test is used to detect the presence of Pneumocystis jirovecii in respiratory specimens using PCR. It is the preferred test for assessing immunocompromised patients who do not have HIV. The results are crucial for diagnosing PCP pneumonia and must be interpreted considering the patient's risk factors, clinical presentation, and radiographic imaging.
Special Instructions
Specimen source must be specified. Bronchoalveolar lavage (BAL), bronchial wash, or induced sputum is preferred. Expectorated sputum is acceptable but not preferred. Use a sterile container for specimen transfer, or alternatively, viral transport media provided by ARUP.
Limitations
A negative result does not exclude the presence of PCR inhibitors in the specimen or the presence of nucleic acid at concentrations below the detection limit of the assay. This test is performed under CLIA regulations but is not FDA approved as it is a laboratory-developed test.
New York Approved
Methodology
PCR-based (Qualitative Polymerase Chain Reaction)
Biomarkers
LOINC Codes
- 31208-2
- 6521-9
Result Turnaround Time
1-5 days
Related Documents
For more information, please review the documents below
Specimen
Bronchoalveolar Lavage
Volume
2 mL
Minimum Volume
0.5 mL
Container
Sterile container or viral transport media
Collection Instructions
Transfer to a sterile container or to viral transport media (ARUP supply #12884).
Storage Instructions
Place each specimen in an individually sealed bag.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 48 hours |
| Refrigerated | 7 days |
| Frozen | 1 month |