Pneumocystis jirovecii DFA with Reflex to Pneumocystis jirovecii by PCR
Also known as: PNEUMST R
Use
This test is used for the rapid identification of Pneumocystis jirovecii. If the initial Direct Fluorescent Antibody (DFA) stain is negative, the test reflexes to PCR testing for confirmation. The test is particularly significant for diagnosing Pneumocystis pneumonia in patients, where sensitivity depends on the patient population and specimen type. It aids in distinguishing Pneumocystis jirovecii infection from other causes of respiratory issues.
Special Instructions
Specimen source is required for test processing. If the initial DFA test is negative, an additional PCR test will be conducted, which incurs additional charges. The test is New York state approved, ensuring its availability for use in New York hospitals and laboratories.
Limitations
A negative DFA result does not exclude the possibility of infection by Pneumocystis jirovecii. False-negative results may occur due to sampling errors or a low number of organisms in the specimen, which can affect the detection capability of both DFA and PCR testing. Formalin-fixed tissues, slides, or swabs are not acceptable for this test, potentially limiting specimen collection options.
New York Approved
Methodology
Immunoassay (Other, PCR-based)
Biomarkers
Result Turnaround Time
1-6 days
Related Documents
For more information, please review the documents below
Specimen
Bronchoalveolar Lavage
Volume
5 mL
Minimum Volume
1 mL
Container
sterile container
Collection Instructions
Collect respiratory specimen: Bronchial washing, bronchoalveolar lavage (BAL), or induced sputum. Transfer 5 mL respiratory specimen to a sterile container.
Storage Instructions
Refrigerate specimen prior to transport. Keep in an individually sealed bag.
Causes for Rejection
Tissues. Specimens in formalin. Slides or swabs.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 2 hours |
| Refrigerated | 1 week |
| Frozen | Unacceptable |