Respiratory Viruses DFA with Reflex to Respiratory Virus Mini Panel by PCR
Also known as: RSPFAPCR
Use
The Respiratory Viruses DFA with Reflex to Respiratory Virus Mini Panel by PCR is used for the detection of respiratory viruses in clinical specimens. The test initially employs an immunofluorescent stain method to detect a range of respiratory viruses. If the DFA (Direct Fluorescent Antibody) result is negative or inadequate, the protocol includes a reflex procedure where a more sensitive Respiratory Virus Mini Panel by PCR (Polymerase Chain Reaction) is added. This test aids in the diagnosis of respiratory tract infections caused by viral pathogens such as influenza viruses, parainfluenza viruses, adenovirus, respiratory syncytial virus (RSV), and human metapneumovirus. It is primarily used in clinical settings to manage and control outbreaks of viral respiratory infections.
Special Instructions
This test is not recommended as a standalone option. It should be used along with a complete clinical evaluation and other diagnostic procedures. If the DFA result is negative or deemed inadequate, the reflex to PCR will incur additional charges. Specimen source information is required for accurate interpretation of results. Storage and transportation of specimens should be conducted according to the guidelines to ensure specimen integrity.
Limitations
The sensitivity of the DFA methodology greatly depends on the adequacy of the collected specimen, particularly the presence of a sufficient number of cells. Specimens containing fewer than 20 cells are reported as inadequate, which may limit the ability to detect viral antigens. PCR inhibitors present in the patient specimen or nucleic acids in concentrations below the detection level of the assay can lead to false-negative results. The test is not FDA cleared or approved but is performed in a CLIA-certified laboratory, compliant for clinical use.
New York Approved
Methodology
Immunoassay (Immunofluorescent Stain / PCR-based)
Biomarkers
LOINC Codes
- 5863-6
- 5867-7
- 5869-3
- 5871-9
- 5873-5
- 5834-7
- 5877-6
- 40979-7
Result Turnaround Time
1 day
Related Documents
For more information, please review the documents below
Specimen
Nasopharyngeal Swab
Volume
3 mL
Minimum Volume
1 mL
Container
viral transport media (ARUP Supply #12884)
Collection Instructions
Nasal aspirate or nasopharyngeal swab should be collected. Fluid: Transfer 3 mL specimen to a sterile container (Min: 1 mL) or to 3 mL viral transport media. Swab: Place in 3 mL viral transport media.
Storage Instructions
Store and transport refrigerated.
Causes for Rejection
Specimens in calcium alginate, eSwab, or dry or wood swabs. Slides.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 2 hours |
| Refrigerated | 72 hours |
| Frozen | Unacceptable |