Casandra

Casandra Test Code AR92315Version 1 (DRAFT)

Performing Lab

Lab ARUP LaboratoriesLab Test ID 2002565CPT Codes 87260, 87275, 87276, 87279, 87280, 87299, 87631

Respiratory Viruses DFA with Reflex to Respiratory Virus Mini Panel by PCR

Also known as: RSPFAPCR

Clinical Use

Use

The Respiratory Viruses DFA with Reflex to Respiratory Virus Mini Panel by PCR is used for the detection of respiratory viruses in clinical specimens. The test initially employs an immunofluorescent stain method to detect a range of respiratory viruses. If the DFA (Direct Fluorescent Antibody) result is negative or inadequate, the protocol includes a reflex procedure where a more sensitive Respiratory Virus Mini Panel by PCR (Polymerase Chain Reaction) is added. This test aids in the diagnosis of respiratory tract infections caused by viral pathogens such as influenza viruses, parainfluenza viruses, adenovirus, respiratory syncytial virus (RSV), and human metapneumovirus. It is primarily used in clinical settings to manage and control outbreaks of viral respiratory infections.

Special Instructions

Not provided.

Limitations

The sensitivity of the DFA methodology greatly depends on the adequacy of the collected specimen, particularly the presence of a sufficient number of cells. Specimens containing fewer than 20 cells are reported as inadequate, which may limit the ability to detect viral antigens. PCR inhibitors present in the patient specimen or nucleic acids in concentrations below the detection level of the assay can lead to false-negative results. The test is not FDA cleared or approved but is performed in a CLIA-certified laboratory, compliant for clinical use.

Test Details

New York Approved

Methodology

Immunoassay (Immunofluorescent Stain / PCR-based)

Biomarkers

2 components

Direct Fluorescent Antibody (DFA) • Immunofluorescent StainRespiratory Virus Mini Panel by PCR • Qualitative Reverse Transcription Polymerase Chain Reaction

LOINC Codes

Result Codes

5863-6 - FLUAV Ag Spec Ql IF
5867-7 - FLUBV Ag Spec Ql IF
5869-3 - HPIV1 Ag Spec Ql IF
5871-9 - HPIV2 Ag Spec Ql IF
5873-5 - HPIV3 Ag Spec Ql IF
5834-7 - HAdV Ag Spec Ql IF
5877-6 - RSV Ag Spec Ql IF
40979-7 - hMPV Ag Spec Ql IF

Result Turnaround Time

1 day

Specimen

Nasopharyngeal Swab

Volume

3 mL

Minimum Volume

1 mL

Container

viral transport media (ARUP Supply #12884)

Collection Instructions

Nasal aspirate or nasopharyngeal swab should be collected. Fluid: Transfer 3 mL specimen to a sterile container (Min: 1 mL) or to 3 mL viral transport media. Swab: Place in 3 mL viral transport media.