RET Gene Rearrangements by FISH
Also known as: RET_FISH
Use
This test is used to detect RET gene rearrangements in solid tumors, specifically in non-small cell lung cancer and thyroid cancer. RET rearrangements occur in approximately 1-2% of lung adenocarcinomas and 10-20% of papillary thyroid carcinomas. Detecting these rearrangements can aid in the diagnostic classification of diseases and predicting tumor response to targeted therapies.
Special Instructions
Not provided.
Limitations
The test detects rearrangements involving the RET gene but does not identify the translocation partner or variant. It does not detect cryptic rearrangements and is intended for specific solid tumor types. Viability is dependent on sufficient tumor tissue being present in the specimen.
New York Approved
Methodology
Chromosomal / Cytogenetics (FISH)
Biomarkers
LOINC Codes
- 81304-8 - Struct var analysis method
- 78236-7 - Cells.IGH rear/Cells cnt NFr Bld/T FISH
Result Turnaround Time
3-7 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
2 slides
Container
Paraffin block or slides
Collection Instructions
Formalin fix (10 percent neutral buffered formalin) and paraffin embed tumor tissue. Transport tissue block or 4 unstained, consecutively cut, 5-micron thick sections, mounted on positively charged glass slides. Protect paraffin block and/or slides from excessive heat.
Storage Instructions
Room temperature. Also acceptable: Refrigerated.
Causes for Rejection
Paraffin block with no tumor tissue remaining. Specimens fixed in any fixative other than 10 percent neutral buffered formalin. Decalcified specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |
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