RhC/c (RHCE) Antigen Genotyping
Also known as: RHC GENO
Use
RhC/c (RHCE) Antigen Genotyping is used to determine the RhC/c genotype of parental or neonatal individuals. This test is crucial for assessing the risk of alloimmune hemolytic disease, which can occur due to erythrocyte alloimmunization resulting in hemolytic transfusion reactions or hemolytic disease of the fetus and newborn (HDFN). By detecting the genotype, it can predict the expression of RhC or Rhc antigens, aiding in understanding the inheritance patterns and potential implications for offspring.
Special Instructions
Informed consent for genetic testing is required for patients residing in New York. Counselors recommend genetic counseling prior to testing. Consent forms are available online or through ARUP Client Services. Inconclusive results may occur in patients who have undergone hematopoietic stem cell transplants. Variations in the Rh locus may occasionally lead to indeterminate results.
Limitations
The assay may not detect rare nucleotide changes that result in altered or partial antigen expression, nor can it assess genotypes leading to Rh null phenotypes. False-negative results for RhC or Rhc predictions might occur due to RHCE-D-CE fusion genes. The assay is sometimes limited in predicting genotype due to extreme variation in the Rh locus, and abnormal signal intensities may also affect genotyping results.
New York Approved
Methodology
PCR-based (PCR)
Biomarkers
LOINC Codes
- 31208-2
- 46731-6
Result Turnaround Time
3-10 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
3 mL
Minimum Volume
1 mL
Container
Lavender (K2EDTA), pink (K2EDTA)
Storage Instructions
Refrigerated.
Causes for Rejection
Yellow (ACD solution A or B); plasma or serum. Specimens collected in sodium heparin tubes. Frozen specimens in glass collection tubes.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 72 hours |
| Refrigerated | 1 week |
| Frozen | 1 month |