ROS1 by FISH
Also known as: ROS1_FISH
Use
This test detects ROS1 gene rearrangements in solid tumors, particularly useful for predicting tumor response to targeted therapy in non-small cell lung carcinomas. It identifies rearrangements involving the ROS1 gene but does not determine the translocation partner or variant. ROS1 rearrangements occur in about 1% of non-small cell lung carcinomas, making this test important for identifying patients who might benefit from targeted treatments.
Special Instructions
Not provided.
Limitations
The test does not identify the translocation partner involved in the rearrangement, nor can it detect specific variants of the ROS1 gene. It may miss cryptic rearrangements and is designed to detect positive signals in 15% or more of tumor cells. The test's limit of detection was established with normal and known positive samples; it provides 100% detection of known ROS1 rearrangements but may not identify all possible rearrangements.
New York Approved
Methodology
Chromosomal / Cytogenetics (FISH)
Biomarkers
No genes
Gene
LOINC Codes
- 59050-5 - Chrom analy interphase Spec FISH-Imp
- 81304-8 - Struct var analysis method
- 78236-7 - Cells.IGH rear/Cells cnt NFr Bld/T FISH
Result Turnaround Time
3-7 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
2 slides
Container
Tissue block or slides in a tissue transport kit
Collection Instructions
Formalin fix (10% neutral buffered formalin) and paraffin embed specimen.
Storage Instructions
Transport at room temperature, or refrigerate during summer.
Causes for Rejection
Paraffin blocks with no tumor tissue remaining, non-formalin fixed, or decalcified specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |
Other tests from different labs that may be relevant