Solid Tumor Mutation Panel, Sequencing

Casandra

Casandra Test Code AR90461Version 1 (DRAFT)

Performing Lab

Lab ARUP LaboratoriesLab Test ID 3004294CPT Code 81445

Solid Tumor Mutation Panel, Sequencing

Also known as: SOLIDNGS

Clinical Use

Order Test

Use

This test is used to assess for targeted variants in solid tumor cancers, including melanoma, gastrointestinal stromal tumor (GIST), colorectal, bladder, and hepatocellular carcinomas. It aids in prognosis and treatment decisions, particularly at initial diagnosis or for refractory disease. If lung cancer is suspected, additional genetic testing for gene rearrangements or c-MET exon 14-skipping alterations may be considered. The test identifies hotspot variants in 44 cancer-related genes, contributing to personalized variant profiles for diagnosis, prognosis, and therapy response predictions.

Special Instructions

Not provided.

Limitations

This test does not detect variants outside the targeted regions or below the detection threshold. It does not identify copy number alterations, translocations, microsatellite instability, or tumor mutational burden. It is not suited to detect minimal residual disease or distinguish somatic from germline variants. Technical limitations in pseudogenes, homologous, and low mapability regions may prevent variant identification in specified exons for certain genes, as large indels over 60bp may also not be detected. Tissue samples yielding fewer than 50ng of DNA may result in suboptimal performance.

Test Details

Methodology

NGS (Targeted)

Biomarkers

Mutation (43 genes)

AKT1ALKAPCATMBRAFCDH1CDKN2ACTNNB1DDR2EGFRERBB2ERBB4EZH2FBXW7FGFR1FGFR2FGFR3GNA11GNAQGNASHRASIDH1IDH2KDRKITKRASMAP2K1METMTORNOTCH1NRASNTRK1PDGFRAPIK3CAPTENRB1RETROS1SMAD4SMOSTK11TP53VHL

LOINC Codes

Order Codes

94736-6 - Tissue block ID Spec

Result Codes

11502-2 - Laboratory report

Result Turnaround Time

12-14 days

Specimen

Tumor Tissue (FFPE)

Volume

Not provided

Minimum Volume

Not provided

Container

Tissue transport kit

Collection Instructions

Transport block and/or slides in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services. Resections: Transport 8 unstained 5-micron slides. (Min: 5 slides). Small Biopsies: Transport 15 unstained 5-micron slides. (Min: 10 slides).

Storage Instructions

Room temperature; Refrigerated also acceptable. Ship in a cooled container during summer.

Causes for Rejection

Less than 10 percent tumor. Specimens fixed in any fixative other than 10 percent neutral buffered formalin. Decalcified specimens. FNA smears with less than 50 tumor cells.