Special Stain, Alcian Blue pH 2.5
Also known as: AB 2.5 SS
Use
This test is a histochemical special stain (Alcian Blue at pH 2.5) designed to identify strongly acidic sulfated mucosubstances in tissue specimens, commonly used to highlight mucopolysaccharides for diagnostic pathology purposes.
Special Instructions
Test is performed as a stain-and-return (technical) service only. Submit electronic request or use ARUP Anatomic Pathology Test Request Form #32960 if electronic ordering is unavailable. Transport specimens in ARUP tissue transport kit #47808 via eSupply or contact client services.
Limitations
Specimens must be representative tissue or cells; depleted or non‑representative tissue is unacceptable. Frozen specimens are unacceptable. Protect paraffin blocks or slides from excessive heat. Slides must be unstained, positively charged, 3–5 microns. Submission limitations include inability to process nonparaffin tissue and requirement for proper fixation and embedding.
New York Approved
Methodology
Other
Biomarkers
LOINC Codes
- 10766-4
Result Turnaround Time
1-5 days
Related Documents
For more information, please review the documents below
Specimen
Tumor Tissue (FFPE)
Volume
Not provided
Minimum Volume
1 slide or block
Container
ARUP tissue transport kit #47808
Collection Instructions
Formalin fix (10 percent neutral buffered formalin preferred), paraffin embed specimen, or prepare cell block; transport tissue block or 2 unstained (3–5 micron) positively charged slides in ARUP kit #47808.
Storage Instructions
Room temperature or refrigerated; ship in cooled container during summer months.
Causes for Rejection
Nonrepresentative tissue type; depleted specimens; frozen specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |