Special Stain, Trichrome
Also known as: TRI SS
Use
Trichrome special stain is utilized in histopathology to differentiate collagen and related tissue components (e.g., collagen, fibrin) and to provide additional morphological detail to support diagnostic interpretation. It is offered as a stain-and-return technical service, enhancing evaluation of tissue fibrosis or muscular/collagen architecture.
Special Instructions
Submit with ARUP Anatomic Pathology Test Request Form (#32960) or via electronic request. Use ARUP supply #47808 Tissue Transport Kit for submission of paraffin block or two unstained (3–5 μm) positively charged slides. Protect slides from heat, transport at room temperature or refrigerated, using cooled container during summer months.
Limitations
Only tissue or cells embedded in paraffin blocks or cellblocks are acceptable; frozen specimens are unacceptable. Unacceptable conditions include nonrepresentative tissue type and depleted specimens. This service is technical only (stain and return), with no interpretive reporting provided by ARUP.
New York Approved
Methodology
Other
Biomarkers
LOINC Codes
- 6473-3
Result Turnaround Time
1-5 days
Related Documents
For more information, please review the documents below
Specimen
Tumor Tissue (FFPE)
Volume
Not provided
Minimum Volume
1 slide (minimum)
Container
tissue block or slides in tissue transport kit (ARUP supply #47808)
Collection Instructions
Formalin fix (10% neutral buffered formalin preferred) and paraffin embed specimen (cells must be prepared into a cellblock). Transport tissue block or 2 unstained (3‑ to 5‑micron thick) positively charged slides in tissue transport kit.
Storage Instructions
Room temperature or refrigerated; ship in cooled container during summer months.
Causes for Rejection
Nonrepresentative tissue type; depleted specimens
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |