TdT by Immunohistochemistry
Also known as: TDT IHC
Use
Terminal deoxynucleotidyl transferase (TdT) immunohistochemistry is used in histopathology to detect TdT expression, a nuclear enzyme typically found in cortical thymocytes, immature hematopoietic stem cells, and lymphoblasts. Its presence aids in the diagnosis and classification of lymphoblastic leukemia or lymphoma, helping to distinguish these from other hematologic malignancies.
Special Instructions
This test is performed as a stain-and-return (technical only) service. Ordering is via electronic request or by using ARUP Immunohistochemistry Stain Form #32978 with an ARUP client number. Specimens must be formalin-fixed, paraffin-embedded tissue or cell blocks, protected from overheating, transported in a tissue transport kit (ARUP supply #47808); precut slides must not be oven baked. During summer months, ship in a cooled container.
Limitations
Unacceptable specimens include non-representative tissue types and depleted specimens. Frozen specimens are unacceptable. Specimens must not be overheated and slides must not have been oven-baked. Frozen submissions are not accepted, and only technical staining is performed; interpretation is not included for this service.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
tissue transport kit (ARUP supply #47808)
Collection Instructions
Transport tissue block or 5 unstained (3- to 5‑micron thick sections), positively charged slides in a tissue transport kit; if sending precut slides, do not oven bake.
Storage Instructions
Protect paraffin block and/or slides from excessive heat; ship in cooled container during summer months.
Causes for Rejection
Non-representative tissue type; depleted specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |