Transforming Growth Factor beta1, Plasma
Also known as: TGFB1 PLA
Use
The Transforming Growth Factor beta1, Plasma test measures levels of TGF-b1 in plasma. TGF-b1 is a cytokine involved in regulating immune and inflammatory responses, and its measurement is useful in research into the pathophysiology of immune or inflammatory disorders. Elevated levels of TGF-b1 may be associated with various medical conditions, including scarring, fibrosis, and certain autoimmune diseases.
Special Instructions
Conventional specimen preparation practices often fail to fully remove platelets from plasma, leading to elevated TGF-b measurements. Following precise centrifugation and immediate freezing is crucial for sample integrity. Specimens must be frozen at temperatures below -20 °C immediately after collection. Separate specimens are required if multiple tests are ordered.
Limitations
Results from this test are intended for research purposes and to understand the pathophysiology of immune or inflammatory disorders. This test is labeled as a Laboratory Developed Test (LDT) performed in a CLIA-certified laboratory and has not been cleared or approved by the U.S. Food and Drug Administration. Conventional specimen preparation practices may lead to elevated TGF-b1 measurements due to incomplete removal of platelets.
New York Approved
Methodology
Immunoassay (ELISA)
Biomarkers
LOINC Codes
- 49853-5
- 49853-5
Result Turnaround Time
1-8 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
1 mL
Minimum Volume
0.3 mL
Container
ARUP standard transport tube
Collection Instructions
Centrifuge anticoagulated whole blood within 2 hours of collection at 1500g for 10 minutes. Collect plasma and transfer to a fresh tube. Immediately centrifuge plasma at 3000g for 10 minutes. Collect plasma from upper 2/3 of tube without disturbing the lower 1/3 and transfer to a fresh tube.
Storage Instructions
Freeze at temperatures below -20 °C immediately after collection. Separate specimens must be submitted when multiple tests are ordered.
Causes for Rejection
Contaminated, severely hemolyzed, heat-inactivated, or grossly lipemic specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 30 minutes |
| Refrigerated | Unacceptable |
| Frozen | 60 days |