Vitamin B1 (Thiamine), Whole Blood
Also known as: VIT B1 WB
Use
This test is used for nutritional assessment of vitamin B1 (thiamine) levels in the body. It measures the concentration of thiamine diphosphate (TDP), the primary active form of vitamin B1, where approximately 90 percent of vitamin B1 in whole blood is TDP. It is significant for patients who need monitoring of thiamine levels due to deficiency, especially in cases of malnutrition, certain medical conditions, or post-bariatric surgery.
Special Instructions
Whole blood is the preferred specimen for thiamine assessment. Use green (sodium or lithium heparin), lavender (EDTA), or pink (K2EDTA) tubes for collection. Freeze the specimen within one hour of collection for valid results.
Limitations
The test measures thiamine diphosphate (TDP) only, which is the primary active form of vitamin B1 in whole blood, accounting for approximately 90 percent. Thiamine and thiamine monophosphate, which make up the remaining 10 percent, are not measured by this assay. Specimens must be whole blood and properly frozen; plasma separator tubes, glass tubes, and clotted or non-frozen specimens are not acceptable.
New York Approved
Methodology
Mass Spectrometry
Biomarkers
LOINC Codes
- 74444-1
Result Turnaround Time
2-5 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
3 mL
Minimum Volume
0.6 mL
Container
Green (sodium or lithium heparin), lavender (EDTA), or pink (K2EDTA) tube
Collection Instructions
Transfer 3 mL whole blood to an ARUP standard transport tube and freeze within 1 hour of collection.
Causes for Rejection
Any specimen other than whole blood. Plasma separator tubes. Glass tubes. Clotted or nonfrozen specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Unacceptable |
| Refrigerated | Unacceptable |
| Frozen | 6 months |