Oncology FISH Analysis - Gain Chromosome 8
Use
The Oncology FISH Analysis - Gain Chromosome 8 is a Fluorescence In Situ Hybridization (FISH) test aimed at identifying chromosome 8 gains associated with acute myeloid leukemia (AML). AML is primarily observed in adults and is characterized by an increase in undifferentiated white blood cells originating from the bone marrow, leading to their entry into the bloodstream. This test is crucial for recognizing full or partial trisomy 8, identified in 10-15% of AML cases, which can signal an intermediate to poor prognosis. However, it must be emphasized that results should always be interpreted alongside relevant clinical and pathological data and should not solely determine an AML diagnosis.
Special Instructions
This test is indicated for patients with clinical or suspected acute myeloid leukemia and for prognostic purposes to identify genetic anomalies. The analysis can be useful if previous cytogenetic tests were inconclusive or to monitor treatment responses.
Limitations
The current FISH assay exclusively detects gains of chromosome 8 and is not designed to identify abnormalities in other genes or chromosomes, which may lead to incomplete diagnoses where additional genetic factors are involved. Therefore, clinical context and additional testing should be considered for a comprehensive evaluation.
Methodology
Chromosomal / Cytogenetics (FISH)
Biomarkers
Result Turnaround Time
3-5 days
Related Documents
For more information, please review the documents below
Specimen
Bone Marrow (Fresh)
Volume
3-5 cc
Minimum Volume
3-5 cc
Container
Sodium Heparin (green-top) tube(s)
Collection Instructions
Draw bone marrow in a Sodium Heparin (green-top) tube(s) and send.
Storage Instructions
Ship at ambient temperature (18-25°C/64-77°F) in an insulated container by overnight courier. Specimen should arrive in the laboratory within 48 hrs of collection. Do not heat or freeze.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 48 hrs |