Adrenocorticotropic Hormone (ACTH), Plasma, Five Specimens
Use
This test is utilized for assessing adrenal gland function, particularly in diagnosing conditions related to abnormal levels of cortisol production such as Addison's disease, Cushing's syndrome, and pituitary tumors. ACTH, produced in the pituitary gland, plays a critical role in stimulating cortisol release from the adrenal glands. The assessment through multiple specimen testing helps to accurately evaluate the dynamic function and response of the adrenal-pituitary axis to various treatments or conditions.
Special Instructions
This test requires the collection of multiple plasma specimens at different times and may involve stimulating the patient with specific drugs or treatments to observe the hormonal response over time. It is critical not to initiate this test unless the patient is properly prepared and any interfering substances, such as biotin, are withheld for at least 72 hours before sample collection. Consult the Endocrine Appendix for detailed collection instructions.
Limitations
High biotin intake can interfere with the test results. Patients consuming biotin supplements should discontinue use at least 72 hours before specimen collection. The test is not advisable if a dynamic hormonal response is not expected or required since multiple specimens taken at precise intervals are essential for accurate analysis.
Methodology
Automated Analyzer (Clinical Chemistry)
Biomarkers
LOINC Codes
- 42598-3
- 46963-5
- 42598-3
- 39564-0
- 12458-6
- 12459-4
- 12460-2
- 46963-5
Result Turnaround Time
1 day
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
The test involves collecting multiple blood specimens at specific intervals after administering a diagnostic dose of medication or other stimuli. Refer to the Endocrine Appendix for more detailed procedures.
Patient Preparation
Patients must abstain from biotin-containing supplements at least 72 hours before sample collection to prevent analytical interference.
Causes for Rejection
Specimens from patients actively consuming biotin within 72 hours prior to collection could lead to rejection due to potential interference.