Allergen Profile, Dog, IgE With Component Reflex
Use
The diagnostic assessment of allergy starts with the patient’s clinical history and examination and is followed by an extract-based analysis to confirm the presence of specific IgE (sIgE) antibody.1,2 sIgE is necessary but not sufficient for eliciting an allergic response and thus generating a definitive diagnosis of allergic disease.1 Testing utilizing allergenic extracts does not lend itself to the differentiation of primary sensitization from a cross-reactivity-driven response because of the complexity of the extracts. Extracts contain most of the extractable allergenic components from the suspected sensitizer. However, it is often not possible to predict the relative risk of having a systemic allergic reaction using an extract-based diagnostic test. Component Resolved Diagnostics (CRD) refers to the diagnostic use of purified or recombinant allergens in the clinical assessment of individuals who suffer reproducible hypersensitivity reactions with exposures to an allergen at a dose tolerated by non-allergic individuals.2-4 This approach offers advantages over the use of a complete extract, especially in polysensitized individuals, given its usefulness for distinguishing between sensitizations specific to singular species and sensitizations due to cross-reactivity.3
Special Instructions
Component resolved diagnostics (CRD) includes testing for specific allergens like Can f 1, Can f 2, Can f 3, Can f 4, Can f 5, and Can f 6 when initial IgE levels to Dog are detected at ≥0.35 kU/L, enabling better distinction between primary sensitization versus cross-reactivity.
Limitations
Allergen-specific IgE assays do not have absolute predictive values for allergic diseases; clinical history must be considered. Although component resolved diagnostics provide detailed allergen evaluation, it cannot replace clinical history and oral food challenge in most cases. Sensitization to unidentified determinants might cause symptoms. Can f 1, Can f 5, and others have defined allergenic roles, but the inherent variability in sensitization and cross-reactivity highlights the complexity of allergy diagnostics.
Methodology
Immunoassay (Multiplex Protein Panel)
Biomarkers
LOINC Codes
- 6098-8
- 8251-1
- 6098-8
- 58773-3
- 58772-5
- 19738-4
- 82543-0
- 64973-1
- 94277-1
Result Turnaround Time
4-6 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.7 mL
Container
Gel-barrier tube
Storage Instructions
Room temperature
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 14 days |
| Refrigerated | 14 days |
| Frozen | 3 months |