Androstenedione, ACTH Stimulation
Also known as: 17-Dione (Two Specimens), δ4-Androstene, 3
Use
This test may involve significant risk and should be performed only by qualified personnel who are familiar with the test and who have taken adequate precautions to protect the safety of the patient. Every effort has been made to ensure accuracy in these recommendations, but clinicians must use their judgement and refer to specific pharmaceutical resources to determine appropriate drug dosages for their patients.
Special Instructions
Blood should be drawn for baseline androstenedione before administering cosyntropin. After injection of cosyntropin (250 μg IM or IV, diluted in 2-5 mL of saline for IV), a second blood sample should be collected 60 minutes later. Use of red-top tubes is required, and specimens need to be transported together with accurate labeling, including patient information and collection details.
Limitations
The test may not provide accurate results if specimens are not handled properly, such as using gel-barrier tubes, which can cause significant absorption and affect results. Factors such as time of day and recent glucocorticoid administration can influence androgen levels and result interpretation. Thus, sample consistency regarding these parameters is crucial for accurate assessments.
Methodology
Mass Spectrometry
Biomarkers
LOINC Codes
- 13857-8
- 24407-9
- 24407-9
- 13857-8
Result Turnaround Time
4-6 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL (each specimen)
Minimum Volume
0.5 mL (each specimen)
Container
Red-top tube
Collection Instructions
Transfer separated serum to a plastic transport tube. Label each tube with the patient's name, collection time, and date. Submit specimens simultaneously on the same test request form.
Storage Instructions
Refrigerate.
Causes for Rejection
Gross hemolysis; lipemia; gel-barrier tube
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 14 days |
| Refrigerated | 14 days |
| Frozen | 14 days |