Apo E Genotyping: Cardiovascular Risk
Use
Evaluate individuals with elevated cholesterol and triglycerides for the E2/E2 genotype associated with type III hyperlipoproteinemia. Aid in the diagnosis of hyperlipoproteinemia.
Special Instructions
This test is intended for evaluation of cardiovascular risk and not for Alzheimer’s disease assessment.
Limitations
The assay is validated only for APOE E2, E3, and E4 alleles. Rare genotypes such as E1/E1, E1/E2, and E1/E3 result in failed results; E2/E4 and E1/E3 cannot be distinguished. Testing does not detect rare pathogenic variants elsewhere in the genome. Potential causes of false positives or negatives include genetic variants, transfusions, bone marrow transplant, mosaicism, technical errors, sample mislabeling, or incorrect family relationship representation.
Methodology
PCR-based (PCR)
Biomarkers
Result Turnaround Time
5-7 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
One adult tube (5 mL)
Minimum Volume
0.5 mL
Container
Lavender-top (EDTA) tube or yellow-top (ACD) tube
Collection Instructions
Invert tube immediately eight to 10 times once tube is filled
Storage Instructions
Maintain at room temperature or refrigerate; stable at room temperature for eight days
Causes for Rejection
Clotted whole blood; incorrect specimen type; frozen whole blood
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 8 days |