BK Virus (BKV), Urine, Quantitative PCR
Also known as: Polyomavirus BK Quantitation, Quantitation of BK Virus DNA PCR, Urine
Use
Detection and quantitation of BK Virus (BKV) in urine stabilized in cobas® PCR Media can be used as an aid in the diagnosis and management of BK virus infections. The assay is traceable to the 1st WHO International Standard for BK Virus Nucleic Acid Amplification Techniques (NAT)-based assays.
Special Instructions
Urine samples must be transferred to the cobas® PCR Urine Sample Kit within 24 hours of collection. Ensure the urine level falls between the two black lines on the cobas® PCR Media tube.
Limitations
The linear range of the assay is 200-100,000,000 IU/mL. The analytical sensitivity of the test is 12.2 IU/mL. The test is unstable if frozen and requires specific handling and storage conditions to maintain its integrity.
Methodology
PCR-based (RT-qPCR)
Biomarkers
LOINC Codes
- 32285-9
- 32285-9
- 90924-2
Result Turnaround Time
3-4 days
Related Documents
For more information, please review the documents below
Specimen
Urine
Volume
Fill urine to fall within the 2 black lines on the cobas® PCR Media tube.
Minimum Volume
Fill urine to fall within the 2 black lines on the cobas® PCR Media tube.
Container
cobas® PCR Media
Collection Instructions
Collect a urine sample in a sterile cup. Use the transfer pipette included in the cobas® PCR Urine Sample Kit to transfer the urine to the cobas® PCR Media tube. Cap the tube tightly and invert 5 times to mix the sample.
Storage Instructions
Submit specimen in cobas® PCR Media tubes under ambient or refrigerated conditions. Do not freeze.
Causes for Rejection
Urine not submitted in cobas® PCR Media; under-filled or over-filled cobas® PCR Media tubes; leaking or broken tube; inadequate storage or transport
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 90 days (stabilized in cobas® PCR Media) |
| Refrigerated | 90 days (stabilized in cobas® PCR Media) |
| Frozen | Unstable |