Chromosome Five-cell Count Plus Microarray (Reveal®), CVS
Also known as: CVS Chromosome Five-cell Count Plus Microarray (Reveal®)
Use
The test allows prenatal detection of chromosomal aneuploidy, and is used to rule out tetraploidy and rearrangements not detected by array, such as balanced translocations and inversions. It also helps to clarify array abnormalities to determine if a structural rearrangement, marker, or isochromosome is present. Array can only provide copy number imbalances and cannot determine structure of an abnormality. The SNP assay will detect chromosomal imbalances that could be associated with developmental delay/congenital anomalies. It provides detection of possible uniparental disomy of any chromosome, the percent and location of homozygosity, including the degree of identity by descent.
Special Instructions
A completed Clinical Questionnaire for SNP Microarray should accompany specimens. Call 800-345-4363 to request the appropriate form, or photocopy the form from Genetics Appendix. Concurrent maternal contamination (MCC) studies (Maternal Cell Contamination [511402]) are recommended. If prior NIPT studies have been performed, include a copy of the report.
Limitations
This SNP assay does not detect balanced rearrangements, low-level mosaicism (<10%), marker chromosomes that only contain heterochromatin, or tetraploidy. This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.
Methodology
Chromosomal / Cytogenetics
Biomarkers
LOINC Codes
- 64089-6
- 64086-2
- 64085-4
- 64087-0
- 33774-1
- 64088-8
- 31208-2
- 48672-0
- 11502-2
- 48000-4
- 62365-2
Result Turnaround Time
10-21 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Unknown)
Volume
20 to 30 mg
Minimum Volume
5 mg for cultured array and chromosomes, 15 mg for direct array and cultured chromosomes
Container
CVS transport tube (a sterile specimen container with transport medium)
Collection Instructions
Specimen is collected in syringe (transabdominal) or catheter (transcervical) and transferred by flushing with the media from the sterile container back into it. After collecting the specimen, wash with sterile saline solution (NaCl 0.95%) containing sodium heparin (two to three drops of sodium heparin in 10 mL of saline). Carefully transfer the specimen into the CVS transport tube using a sterile Pasteur pipette or a sterile fine needle forceps. Be sure to fill transport tubes completely with medium. Samples from twin (multiple) pregnancies should be appropriately labeled and placed in separate transport containers with a separate test request form for each twin.
Storage Instructions
Maintain specimen at room temperature, and transport it to the cytogenetics laboratory immediately. Do not freeze. Refrigerate if sterility is questioned or if sample cannot be shipped within 24 hours.
Causes for Rejection
No villi submitted in specimen; improperly labeled specimen; frozen specimen or specimen placed in fixative.